Screen Quest Luminometric Calcium Assay Kit
货号： 36305 产品名称： Screen Quest Luminometric Calcium Assay Kit 品牌： AAT 规格： 10 Plates 二周到货 生化试验
Screen Quest� Luminometric Calcium Assay Kit
Cat # 36305
Storage F/D/L (see storage codes below)
Unit Size 10 Plates
Ex (nm) 437
Em (nm) 466
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine朾ased kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
36306 Screen Quest Luminometric Calcium Assay Kit *10X10 Plates* 品牌 AAT
Screen Quest Luminometric Calcium Assay Kit *10X10 Plates*
货号： 36306 产品名称： Screen Quest Luminometric Calcium Assay Kit *10X10 Plates* 品牌： AAT 规格： 100 Plates 二周到货 生化试验
Screen Quest� Luminometric Calcium Assay Kit *10X10 Plates*
Cat # 36306
Storage F/D/L (see storage codes below)
Unit Size 100 Plates
Ex (nm) 437
Em (nm) 466
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine朾ased kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
11559 Amplite Luminometric Peroxidase Assay Kit 品牌 AAT
Amplite Luminometric Peroxidase Assay Kit
货号： 11559 产品名称： Amplite Luminometric Peroxidase Assay Kit 品牌： AAT 规格： 1 kit 二周到货 生化试验
Amplite� Luminometric Peroxidase Assay Kit
Cat # 11559
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 425
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. Peroxidase is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc. The kit provides an optimized 憁ix and read� assay protocol that is compatible with HTS liquid handling instruments.
Detailed Information: Protocol References MSDS
Related Products: Horseradish Peroxidase (HRP) Reactive Oxygen Species Miscroplate Readers
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
11956 Amplite Luminometric Alkaline Phosphatase Assay Kit *Luminescence* 品牌 AAT
Amplite Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
货号： 11956 产品名称： Amplite Luminometric Alkaline Phosphatase Assay Kit *Luminescence* 品牌： AAT 规格： 1 kit 二周到货 生化试验
Amplite� Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
Cat # 11956
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm)
Em (nm) 560
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite� Alkaline Phosphatase Assay Kit uses a proprietary luminogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions as well as in cell extracts. This proprietary phosphatase substrate generates a luminescent product that produces strong luminescence upon interaction with phosphatase. The kit provides all the essential components with our optimized 憁ix and read� assay protocol that is compatible with HTS liquid handling instruments. It has extremely high sensitivity, and can be used for the assays that require demanding sensitivity.
Detailed Information: Protocol References MSDS Spectrum
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21609 PhosphoWorks Luminometric ATP Assay Kit *Steady Glow* 品牌 AAT
PhosphoWorks Luminometric ATP Assay Kit *Steady Glow*
货号： 21609 产品名称： PhosphoWorks Luminometric ATP Assay Kit *Steady Glow* 品牌： AAT 规格： 1 kit 二周到货 生化试验
PhosphoWorks� Luminometric ATP Assay Kit *Steady Glow*
Cat # 21609
Storage F/D/L (see storage codes below)
Unit Size 1 Plate
Em (nm) 560
Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling. The PhosphoWorks� ATP Assay Kit provides a fast, simple and homogeneous luminescence assay for the determination of cell proliferation and cytotoxicity in mammalian cells. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The high sensitivity of this assay permits the detection of ATP in many biological systems, environmental samples and foods. This PhosphoWorks ATP Assay Kit has the stable luminescence signal as long as 4 hours. It has stable luminescence with no mixing or separations required, and formulated to have minimal hands-on time.
Detailed Information: Protocol References MSDS
Related Products: Anions Cell Cytotoxicity Protein Kinases
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21610 PhosphoWorks Luminometric ATP Assay Kit *Bright Glow* 品牌 AAT
PhosphoWorks Luminometric ATP Assay Kit *Bright Glow*
货号： 21610 产品名称： PhosphoWorks Luminometric ATP Assay Kit *Bright Glow* 品牌： AAT 规格： 1 kit 二周到货 生化试验
PhosphoWorks� Luminometric ATP Assay Kit *Bright Glow*
Cat # 21610
Storage F/D/L (see storage codes below)
Unit Size 1 Plate
Em (nm) 560
Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling. The PhosphoWorks� ATP Assay Kit provides a fast, simple and homogeneous luminescence assay for the determination of cell proliferation and cytotoxicity in mammalian cells. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The high sensitivity of this assay permits the detection of ATP in many biological systems, environmental samples and foods. This PhosphoWorks ATP Assay Kit can detect as low as 10 cells/well. It has stable luminescence with no mixing or separations required, and formulated to have minimal hands-on time.
Detailed Information: Protocol References MSDS
Related Products: Anions Cell Cytotoxicity Protein Kinases
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)