标签： cdkrap

Anti-CDK5RAP2

货号： 06-1398      产品名称： Anti-CDK5RAP2   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-CDK5RAP2
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REFERENCES
Loss of centrioles causes chromosomal instability in vertebrate somatic cells.
Sir, Joo-Hee, et al. (2013) J. Cell Biol., (2013)
CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response.
Barr, Alexis R, et al. (2010) J. Cell Biol., 189: 23-39 (2010)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-CDK5RAP2 Antibody
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Molecular Weight:
~220 kDa observed. Uniprot.org states four isoforms produced by alternative splicing, which may be observed at a molecular weight of ~216 kDa, ~211 kDa, ~210 kDa, and ~206 kDa in some cell lysates.
Immunogen:
MBP-tagged recombinant protein corresponding to human CDK5RAP2.
Background Information:
Also known as Cep215, CDK5RAP2 is involved in a variety of cellular processes. It serves as a potential regulator of CDK5 activity through its interaction with CDK5R1. It acts as a negative regulator of centriole disengagement, which maintains centriole engagement and cohesion. It is involved in the regulation of mitotic spindle orientation. Additionally, this protein mediates spindle checkpoint activation by serving as a transcriptional regulator of the MAD2 and BUBR1 promoter. In conjuction with MAPRE1, CDK5RAP2 promotes microtubule polymerization, bundle formation, and growth at the plus ends. It also plays a role in DNA damage-induced G2 cell cycle checkpoint. CDK5RAP2 is known to be mutated in primary microcephaly, a neuodevelopmental disorder that is characterized by a decrease in brain size.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Other Homologies: Rat (83% sequence homology) and Mouse (82% sequence homology).
Application Notes:
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected CDK5RAP2 in RPE1 cells. (Barr, A.R., et al. (2010). J Cell Biol. 189(1):23-39.)
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:2,500 dilution of this antibody dectected CDK5RAP2 in 10µg of HeLa cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q96SN8
Entrez Gene Number:
NP_001011649
Gene Symbol:
CDK5RAP2
Alternate Names:
CDK5 regulatory subunit-associated protein 2
CDK5 activator-binding protein C48
Centrosome-associated protein 215
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein may also be a substrate of neuronal CDC2-like kinase. Multiple 2°C – 8°C