WNT-1, human recombinant

货号： 4754-50      产品名称： WNT-1, human recombinant   品牌： Biovision 规格： 50 μg 3周到货 生化实验

WNT-1, human recombinant
A proto-oncogene implicated in oncogenesis and in several developmental processes
Product Overview
Alternate Names / Synonyms: Human WNT-1, WNT-1, WNT1, WNT 1, h-WNT-1, rh-WNT-1, recombinant human WNT-1, recombinant WNT-1, WNT
Gene Symbol: WNT1
Accession #: P04628
Gene ID: 7471
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized with no additives
Molecular Weight: 38.4 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED₅₀ for this effect is 1.5 – 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers
Storage Temp.: -20°C
Shipping: Blue ice
Background Information: Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intraCellular signaling protein, β-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C