Pro-MMP-2, mouse

货号： 7780-5      产品名称： Pro-MMP-2, mouse   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Pro-MMP-2, mouse
A Protein involved in breakdown of extracellular matrix
Product Overview
Alternate Name/Synonyms: MMP-2, matrix metalloproteinase-2, MMP, MMP recombinant, MMP protein
Gene Symbol: MMP2
Accession #: Q3UG07
Gene ID: 17390
Source: mouse fibroblasts
Appearance: Liquid
Physical Form Description: In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl₂; 1 μM ZnCl₂; 0.05% Brij 35; 0,05% NaN₃
Molecular Weight: 40/42 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry Ice
Background Information: The progelatinase A, a member of the matrix metalloproteinase (MMP) family, has been isolated from macrophages and fibroblasts. Gelatinase A hydrolyses several components of the extracellular matrix, e.g. the collagen types IV, V and XI and gelatin. Progelatinase A complexed via their C-terminal domain with TIMP-2 was isolated from culture media of different cell types. This complex shows both properties of its constituents: Like TIMP-2 it inhibits active matrix metalloproteinases and like gelatinase it shows proteolytic activity after activation with APMA (4-aminophenylmercury acetate). However, its proteolytic activity is less than 10% of that of gelatinase A not complexed with TIMP-2. In contrast to the other MMPs the progelatinase A cannot be activated by the serine proteinase trypsin. Until quite recently a potential natural activator that can transform latent progelatinase A into the active form was unknown. It was shown that the catalytic domain of the membrane type 2-matrix metalloproteinase activates progelatinase A as well as the progelatinase A / TIMP-2 complex, by cleaving the 72 kDa progelatinase A to yield 67 kDa gelatinase A, which is than transformed into 62 kDa gelatinase A. The 62 kDa form is about twice as active as the 67 kDa form towards the Dnp-pepitde (Masui et al.). No significant difference in activity was found between free and complexed gelatinase A forms
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-80°C