Anti-IDH2

货号： ABC57      产品名称： Anti-IDH2   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-IDH2 Antibody
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-IDH2 Antibody
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Molecular Weight:
~50 kDa observed
Immunogen:
KLH-conjugated linear peptide corresponding to human IDH2.
Background Information:
Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) is a 452 amino acid enyzme belonging to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains mitochondrial glutathione levels. Studies suggest that IDH2 plays a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with Human and Mouse.
Application Notes:
Western Blot Analysis: A previous lot of this antibody detected IDH2 in human MOLT-4 cell lysate.
Control:
Mouse heart tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse heart tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected IDH2 in 10 µg of mouse heart tissue lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P48735
Entrez Gene Number:
NP_002159
Gene Symbol:
IDH2
Alternate Names:
Isocitrate dehydrogenase [NADP], mitochondrial
IDH
ICD-M
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq, Jul 2008].
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UniProt Summary:
FUNCTION: Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
CATALYTIC ACTIVITY: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
SUBUNIT STRUCTURE: Homodimer.
SUBCELLULAR LOCATION: Mitochondrion.
INVOLVEMENT IN DISEASE: Defects in IDH2 are the cause of D-2-hydroxyglut 2-8℃