Anti-PDE4D3

货号： ABN117      产品名称： Anti-PDE4D3   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-PDE4D3
Species Reactivity Key Applications Host Format Antibody Type
M, R, Rhesus Macaque  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-PDE4D3 Antibody
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Specificity:
This antibody recognizes PDE4D3 at the N-terminus.
Molecular Weight:
~95 kDa observed. UniProt describes 12 isoforms produced by alternative splicing.
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminus of human PDE4D3.
Background Information:
Phosphodiesterase 4D3 (PDE4D3) is a member of the PDE cyclic nucleotides. PDE4D3 is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D3 is a direct target of the MSH/cAMP/MITF pathway and may play a role in melanocyte differentiation. PDE4D3 is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D3 may be associated with analgesic and anti-inflammatory effects. PDE4D3 has also been linked to the risk of stroke, and in particular to polygenic stroke.
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Species Reactivity:
Mouse
Rat
Rhesus Macaque
Species Reactivity Note:
Demonstrated to react with Mouse and Rat. Predicted to react with Rhesus Monkey based on 100% sequence homology. Predicted to react with Human based on immunogen design.
Control:
Mouse brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PDE4D3 on 10 µg of mouse brain membrane tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q08499
Entrez Gene Number:
NP_006194
Gene Symbol:
PDE4D
DPDE3
Alternate Names:
cAMP-specific 3′,5′-cyclic phosphodiesterase 4D
DPDE3
PDE43
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes one of four mammalian counterparts to the fruit fly ‘dunce’ gene. The encoded protein has 3′,5′-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.
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UniProt Summary:
FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
CATALYTIC ACTIVITY: Adenosine 3′,5′-cyclic phosphate + H2O = adenosine 5′-phosphate.
COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by rolipram. Activated by phosphatidic acid.
PATHWAY: Purine metabolism; 3′,5′-cyclic AMP degradation; AMP from 3′,5′-cyclic AMP: step 1/1.
SUBUNIT STRUCTURE: Homodimer for the long isoforms. Isoforms with truncated N-t 2-8℃