FlowCellectMouse Th2 Intracellular Cytokine Kit,2-8C
货号： FCIM025124-1 产品名称： FlowCellectMouse Th2 Intracellular Cytokine Kit,2-8C 品牌： Millipore 规格： *** 三周到货 生化实验
FlowCellect™ Mouse Th2 Intracellular Cytokine Kit
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PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore’s Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore’s proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore’s line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ Mouse Th2 Intracellular Cytokine Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Mouse Th2 Identification Kit is designed to enable a researcher a quick and easy way to detect IL-4 expression in mouse Th2 CD4+ T-cells.
Millipore’s FlowCellect™ Mouse Th2 Identification Kit contains a PE conjugated antibody to IL-4 and a PerCP conjugated antibody to CD4 as well as optimized cytokine blocking reagents and buffers to aid in identifying Th2 CD4+ T-cell subsets in ex vivo lymphocyte populations or to monitor Th2 CD4+ T-cell differentiation in culture. In addition, Millipore has added fixable viability dye that eliminates false positive data resulting from cytokine staining on dead cells that can occur in long term cultures. The Millipore’s FlowCellect™ Mouse Th2 Identification Kit contains sufficient reagents for 25 3-color tests. Detailed assay instructions are included to assist in analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data. This kit is not designed to be used in the analysis of whole spleen cells in SJL mice.
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Background Information:
There are five main subsets of differentiated CD4+ T-cells; Th1, Th2, Th17, Treg and TFh cells. Each subtype expresses a signature cytokine or transcription factor that directs the immune response and acts to regulate differentiation. TH2 CD4+ T-cells provide protection against extracellular parasites but have also been shown to cause heightened allergic responses to develop. They mediate immune responses to extracellular pathogens and respond by producing interleukin 4 (IL-4). Through secretion of IL-4, Th2 CD4+ T-cells activate B-cells to develop into IgE-secreting cells and thus activating basophil/mast cell to release histamine, a major contributor to allergic responses. IL-4 also increases the production of IL-4 by Th2 CD4+ T-cells, thereby promoting the Th2 profile.
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Applications:
This FlowCellect Mouse Th2 Identification Kit for flow cytometry is designed to enable a researcher a quick & easy way to detect IL-4 expression in mouse Th2 CD4+ T-cells.
Key Applications:
Flow Cytometry
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses 4-8°C
