Anti-GFAP (N-term), clone EPR1034Y

货号： 04-1062      产品名称： Anti-GFAP (N-term), clone EPR1034Y   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-GFAP (N-term), clone EPR1034Y, Rabbit Monoclonal
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REFERENCES
Chronic-pain-associated astrocytic reaction in the spinal cord dorsal horn of human immunodeficiency virus-infected patients.
Yuqiang Shi,Benjamin B Gelman,Joshua G Lisinicchia,Shao-Jun Tang (2012) The Journal of neuroscience : the official journal of the Society for Neuroscience.32
Regulation of Wnt signaling by nociceptive input in animal models.
Yuqiang Shi,Subo Yuan,Bei Li,Jigong Wang,Susan M Carlton,Kyungsoon Chung,Jin Mo Chung,Shao-Jun Tang (2012) Molecular pain.8
Synthesis and resolution of cis-(+/-)-methyl (1R,2S/1S,2R)-2-[(4-hydroxy-4-phenylpiperidin-1-yl)methyl]-1-(4-methylphenyl)cyclopropanecarboxylate [(+/-)-PPCC)]: new sigma receptor ligands with neuroprotective effect.
Prezzavento, Orazio, et al. (2010) J. Med. Chem., 53: 5881-5 (2010)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IH(P), ICC, IP Rabbit Unpurified Monoclonal Antibody
Description:
Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal
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Specificity:
This antibody recognizes GFAP at and around the N-terminus.
Molecular Weight:
~50 kDa
Epitope:
N-terminus
Immunogen:
Synthetic peptide corresponding to residues in the N-terminus of human GFAP.
Clone:
EPR1034Y
Isotype:
IgG
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse, and rat.
Application Notes:
Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected GFAP in human brain and human glioma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.
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Control:
Human and Rat brain lysates
Quality Assurance:
Evaluated by Western Blot on Human and Rat brain cell lysates.
Western Blot Analysis: 1:50,000-100,000 dilution of this antibody was used to detect GFAP in human and rat brain cell lysates.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P14136
Entrez Gene Number:
NP_001124491.1
Gene Symbol:
FLJ45472
Alternate Names:
glial fibrillary acidic protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or applica -20°C