ANTI-EMT/ITK/TSK PC 12-303
货号： 05-476 产品名称： ANTI-EMT/ITK/TSK PC 12-303 品牌： Millipore 规格： EA 3周到货 生化实验
Anti-Itk/Emt/Tsk Antibody, clone 2F12
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REFERENCES
Biochemical interactions integrating Itk with the T cell receptor-initiated signaling cascade.
Bunnell, S C, et al. (2000) J. Biol. Chem., 275: 2219-30 (2000)
Regulated association between the tyrosine kinase Emt/Itk/Tsk and phospholipase-C gamma 1 in human T lymphocytes.
Perez-Villar, J J and Kanner, S B (1999) J. Immunol., 163: 6435-41 (1999)
T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells.
Liu, K Q, et al. (1998) J. Exp. Med., 187: 1721-7 (1998)
Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity.
Heyeck, S D, et al. (1997) J. Biol. Chem., 272: 25401-8 (1997)
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Species Reactivity Key Applications Host Format Antibody Type
H, M IP, WB Mouse Ascites Monoclonal Antibody
Description:
Anti-Itk/Emt/Tsk Antibody, clone 2F12
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Trade Name:
Upstate (Millipore)
Specificity:
Itk
Molecular Weight:
72kDa
Immunogen:
GST fusion-protein corresponding to residues 1-26 of Itk/Emt/Tsk
Isotype:
IgG1
Background Information:
ITK is a T cell signaling protein. The Tec family of non receptor tyrosine kinases is composed of six proteins designated Tec, ITK (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. ITK associates with CD28 and becomes activated subsequent to CD28 ligation.
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Species Reactivity:
Human
Mouse
Control:
Jurkat cell lysate, K562 and A431 cell lysates, normal lymph node tissue
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Purification Method:
Ascites
Presentation:
PBS, containing 0.02% sodium azide
Storage Conditions:
2 years at -20°C
UniProt Number:
Q08881
Entrez Gene Number:
NM_005546
Gene Symbol:
ITK
LYK
PSCTK2
EMT
MGC126258
MGC126257
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
UniProt Summary:
FUNCTION: SwissProt: Q08881 # Plays a role in T-cell proliferation and differentiation.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SIZE: 620 amino acids; 71831 Da
SUBCELLULAR LOCATION: Cell membrane (By similarity). Note=Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T- cells (By similarity).
TISSUE SPECIFICITY: T-cell lines and natural killer cell lines.
SIMILARITY: SwissProt: Q08881 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC -20°C
