ANTI-ITK
货号： 06-1064 产品名称： ANTI-ITK 品牌： Millipore 规格： EA 3周到货 生化实验
Anti-Itk/Emt/Tsk
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Itk/Emt/Tsk Antibody
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Specificity:
Recognizes Itk/Emt/Tsk, Mr ~72 kDa.
Molecular Weight:
72 kDa
Immunogen:
Recombinant protein.
Itk/Emt/Tsk-GST fusion protein (Itk/Emt/Tsk amino acid residues 1-270) spanning the PH, SH3 domains and a small portion of the SH2 domain; bound to nitrocellulose paper.
Background Information:
Itk is a T cell signaling protein. The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Itk (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. Itk associates with CD28 and becomes activated subsequent to CD28 ligation.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human. Predicted to cross-react with mouse based on 100% sequence homology.
Control:
Jurkat cell lysates
Quality Assurance:
Routinely evaluated by western blot on Jurkat cell lysate.
Western Blot Analysis:
0.05-1 µg/mL of this lot detected Itk/Emt/Tsk in 10 µg of a Jurkat cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polycloncal in buffer containing PBS with 0.05% sodium azide and 50% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial and prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
Q08881
Entrez Gene Number:
NP_005537
Gene Symbol:
EC 2.7.10.2
EMT
LYK
MGC126257
MGC126258
PSCTK2
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Alternate Names:
IL2-inducible T-cell kinase
Kinase EMT
T-cell-specific kinase
Tyrosine-protein kinase Lyk
homolog of mouse T-cell itk/tsk
tyrosine-protein kinase ITK/TSK
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq]
Product Name:
Anti-Itk/Emt/Tsk
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect Itk/Emt/Tsk using this Anti-Itk/Emt/Tsk -20°C
