Anti-IRAK-M (rabbit polyclonal)

货号： 07-1481      产品名称： Anti-IRAK-M (rabbit polyclonal)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-IRAK-M Antibody, a.a. 581-596
Recommended Replacement for: 07-1481 is a recommended replacement for AB16534
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IH(P) Rabbit Purified Polyclonal Antibody
Description:
Anti-IRAK-M Antibody, a.a. 581-596
Replaces:
07-1481 is a recommended replacement for AB16534
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Specificity:
This antibody recognizes IRAK-M at a.a. 581-596.
Molecular Weight:
~68 kDa
Epitope:
a.a. 581-596
Immunogen:
Rabbit anti-IRAK-M polyclonal antibody was raised against a peptide corresponding to amino acids 581 to 596 of human IRAK-M (3).
Isotype:
IgG
Background Information:
IRAK (IL-1 receptor associated kinase) is a serine/threonine kinase which associates with the IL-1 receptor after ligand binding to initiate a signaling cascade that ultimately results in nuclear localization of NFκB thought to be important for inflammation. Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF- κB by IL-1/Toll receptors (1,2), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M (3). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1and LPS.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse, and rat reactive.
Application Notes:
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of IRAKm With Citrate pH 6.0 Epitope Retrieval: Human Kidney
Western blot: 0.5 to 1.0 µg/mL of a previous lot was used on mouse spleen or rat kidney tissue lysate can be used as positive control and a 68 kDa major band can be detected.
Control:
A431 Cell Lysate, mouse spleen or rat kidney tissue lysate
Quality Assurance:
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected IRAK on 10 μg of A431 lysates.
Purification Method:
Purified
Presentation:
Purified rabbit polyclonal IgG in PBS containing 0.02% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
"Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q9Y616
Entrez Gene Number:
NM_007199.1
Gene Symbol:
IRAK3
IRAK-3
IRAKM
ASRT5
IRAK-M
EC 2.7.11.1
Alternate Names:
IL- 1 receptor-associated kinase M
Interleukin-1 receptor-associated kinase 3
Interleukin-1 receptor-associated kinase M
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic u 2-8℃