Enhanced Green Fluorescent Protein (EGFP)
货号: 4999-5000 产品名称: Enhanced Green Fluorescent Protein (EGFP) 品牌: Biovision 规格: 5 mg 3周到货 生化实验
Enhanced Green Fluorescent Protein (EGFP)
Fluorescent protein ideal for subcellular labeling/visualization
Product Overview
Alternate Names / Synonyms: Recombinant EGFP Protein, EGFP Protein, h-EGFP Protein, rh-EGFP Protein, recombinant human EGFP Protein, EGFP
Gene Symbol:
Accession #:
Gene ID:
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Freeze Dried
Molecular Weight: 32.7 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: ≥97%
Endotoxin Level: <0.1 ng/μg
Biological Activity: N/A
Reconstitution Instructions: Reconstitute with PBS to 1 mg/ml or other appropiate buffer
Storage Temp.: -80°C
Shipping: Gel pack
Background Information: The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (Gene Bank Accession no. U57607) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody (Cat.# 3998-100) and GFP antibody (Cat.# 3999-100) on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm (ε489 nm = 55,000 M⁻ˡ Cm⁻ˡ; ε280 nm = 21980 M⁻ˡ Cm⁻ˡ).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
-80°C
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