MAPKAPK3, Active

货号： 7755-5      产品名称： MAPKAPK3, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active MAPKAPK3
Involved in many cellular processes
Product Overview
Alternate Names / Synonyms: Active MAPKAPK3, MAPKAPK3, MAPKAPK, human MAPKAPK3, active kinase, active kinases, recombinant MAPKAPK3, recombinant MAPKAPK3 kinase activity assay, protein kinase
Gene Symbol: MAPKAPK3
Accession #: Q16644
Gene ID: 7867
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 69.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 636 nmol phosphate incorporated into MBP protein per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: MAPKAPK3 has a single potential SH3-binding site in the proline-rich N terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK2 (1). MAPKAPK3 was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses (2). This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation (3). MAPKAPK3 is uniquely poised to support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB (4).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C