Anti-RIG-I (N-Terminus)

货号： 06-1040      产品名称： Anti-RIG-I (N-Terminus)   品牌： Millipore 规格： *** 3周到货 生化实验

Anti-RIG-I (N-Terminus)
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REFERENCES
A functional C-terminal TRAF3-binding site in MAVS participates in positive and negative regulation of the IFN antiviral response.
Suzanne Paz,Myriam Vilasco,Steven J Werden,Meztli Arguello,Deshanthe Joseph-Pillai,Tiejun Zhao,Thi Lien-Anh Nguyen,Qiang Sun,Eliane F Meurs,Rongtuan Lin,John Hiscott (2011) Cell research.21
Species Reactivity Key Applications Host Format Antibody Type
H  WB, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-RIG-I (NT) Antibody
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Specificity:
This antibody recognizes RIG-I at the N-terminus.
Molecular Weight:
~102 kDa observed
Epitope:
N-terminus
Immunogen:
GST-tagged recombinant protein corresponding to the N-terminus of human RIG-I.
Background Information:
Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Other homologies: Rhesus Monkey (99% sequence homology).
Application Notes:
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Paz, S., et al. (2011). Cell Research. 1-16.)
Control:
SeV treated and untreated A549 cell lysates
Quality Assurance:
Evaluated by Western Blot in SeV treated and untreated A549 cell lysates.
Western Blot Analysis: 0.05 µg/mL of this antibody detected RIG-I on 10 µg of SeV untreated and treated A549 cell lysates.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O95786
Entrez Gene Number:
NP_055129.2
Gene Symbol:
DDX58
Alternate Names:
Probable ATP-dependent RNA helicase DDX58
DEAD box protein 58
Retinoic acid-inducible gene 1 protein
RIG-1
Retinoic acid-inducible gene I protein
RIG-I
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune 2°C – 8°C