psiTEST System

货号： ksitest      产品名称： psiTEST System   品牌： Invivogen 规格： 1 kit 三周到货 生化试验

psiTEST™ System
Prevalidation of siRNAs and shRNAs
The psiTEST™ System was developed to provide a rapid, simple, and convenient method to screen for functional siRNA and shRNA sequences. The psiTEST™ system can be used to evaluate RNAi on virtually any gene of any species for which the sequence is known. The silencing efficiency of a given siRNA or shRNA is evaluated by monitoring the reduction of expression of a chimeric gene consisting of a secreted alkaline phosphatase (SEAP) reporter gene transcriptionally fused to the target gene. Transfection of effective siRNAs or shRNAs triggers the RNAi process resulting in the degradation of the chimeric mRNA and therefore the absence of SEAP activity (see procedure in "Details" tab).
The psiTEST™ System comprises two components:
– psiTEST™ plasmid featuring the SEAP reporter gene and the GFP::zeo fusion gene.
– QUANTI-Blue™, a SEAP detection medium
The psiTEST™ plasmid can be used to clone fragments of genes or entire genes, the optimum size being 1.5 kb, although genes up to 3.5 kb can be targeted.
The psiTEST™ plasmid is selectable with Zeocin in E. coli and mammalian cells. Transfection efficicency can be monitored by assessing GFP expression.