MMP-2, PROENZYME, HUMAN, RECOMBINANT

货号： PF037-10UG      产品名称： MMP-2, PROENZYME, HUMAN, RECOMBINANT   品牌： Merck 规格： *** 3周到货 生化实验

MMP-2, Proenzyme, Human, Recombinant, CHO Cells
72 kDa Gelatinase, Matrix Metalloproteinase 2, Gelatinase A
Recombinant, human pro-MMP-2 expressed in CHO cells. Can be used as a positive control for zymographic analysis, immunoblotting, and substrate cleavage assay. A portion (less than 10%) of the enzyme may be seen as a naturally-occurring dimer. Dimerization does not interfere with activation. May contain up to 10% TIMP proteins. During storage, a small portion (less than 10%) of the enzyme may also become activated.
产品信息
Form  Liquid
Formulation  In 150 mM NaCl, 20 mM Tris-HCl, 5 mM CaCl2, 0.05% BRIJ®-35 Detergent, pH 7.4.
Preservative  None
Molar mass  72000
Avoid freeze/thaw  是的
Activity  Measured by its ability to degrade gelatin using zymography. 1 ng of enzyme is sufficient to visualize degraded gelatin by coomassie blue stain in zymography. Activated rhMMP-2 degrades type IV collagen, as visualized by SDS-PAGE and cleaves the coumarin peptide substrate, (7-methoxycoumarin-4-yl) Acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl-L-2, 3-diaminopropionyl)-Ala-Arg-NH2 (Cat. No. 03-32-5032), as measured by fluorescence (excitation at 320 nm and emission at 405 nm).
Purity  ≥90% by SDS-PAGE
EC number  3.4.24.24
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling

≤ -70°C