Anti-phospho-Rsk3 (Thr353/Thr356), clone E202
货号： 04-1125 产品名称： Anti-phospho-Rsk3 (Thr353/Thr356), clone E202 品牌： Millipore 规格： EA 3周到货 生化实验
Anti-phospho-Rsk3 (Thr353/Thr356), clone E202, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC, IH(P), IP Rabbit Unpurified Monoclonal Antibody
Description:
Anti-phospho-Rsk3 (Thr353/Thr356) Antibody, clone E202, rabbit monoclonal
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Specificity:
The antibody only detects Rsk phosphorylated on Thr353 and Thr356.
Molecular Weight:
~90 kDa
Epitope:
Phosphorylated Thr353/Thr356
Immunogen:
Phospho-synthetic peptide corresponding to residues surrounding Thr353 and Thr356 of human Rsk3.
Modifications:
Phosphorylation
Clone:
E202
Isotype:
IgG
Background Information:
Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90 kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse, and rat.
Application Notes:
Western Blot Analysis: A 1:5,000-20,000 dilution from a previous lot was used in WB.
Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:100 dilution from a previous lot was used in IP.
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Control:
Human prostate carcinoma tissue
Quality Assurance:
Evaluated by Immunohistochemistry on human prostate carcinoma.
Immunohistochemistry Analysis: A 1:50-100 (antigen retrieval required) dilution of this antibody was used to detect Rsk3 in prostate carcinoma tissue.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q15349
Entrez Gene Number:
NP_001006933.1
Gene Symbol:
EC 2.7.11.1
HU-2
MAPKAPK1C
RSK
RSK-3
RSK3
S6K-alpha
S6K-alpha2
p90-RSK3
pp90RSK3
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Alternate Names:
90 kDa ribosomal protein S6 kinase 2
MAP kinase-activated protein kinase 1c
Ribosomal S6 kinase 3
S6K-alpha 2
p90-RSK 2
ribosomal protein S6 kinase alpha 2
ribosomal protein S6 kinase, 90kD, polypeptide 2
ribosomal protein S6 kinase, 90kDa, polypeptide 2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or -20°C
