SUMO-1 Activating Enzyme (human) (recombinant)
货号： ALX-201-090-C010 产品名称： SUMO-1 Activating Enzyme (human) (recombinant) 品牌： Alexis 规格： 10.micro.g 三周 生化实验
SUMO-1 activating enzyme (human), (recombinant)
Activating enzyme for sumoylation assays.
Product Specification
Alternative Name: SAE1/SAE2
Source: Produced in E. coli. Heterodimer of SAE1 (aa 1-347) and SAE2 (aa 1-640).
UniProt ID: Q9UBE0 (subunit 1), O95605 (subunit 2)
Concentration: 0.2mg/ml
Formulation: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 1mM DTT and 200mM potassium chloride.
Long Term Storage: -80°C
Miscellaneous/General: SAE1/SAE2 is a heterodimer that acts as the activating enzyme for SUMO-1, SUMO-2 and SUMO-3 and catalyzes formation
of a thioester bond between the C-terminus of SUMO/sentrin (aa 1-347) and a cysteine residue in SAE2 (aa 1-640). In
the presence of Ubc9, SAE1/SAE2 transfers the activated SUMO to a cysteine residue in Ubc9.
Background / Technical Information: Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating
enzyme (Prod. No. ALX-201-090) (100ng), UbcH9 (Prod. No. ALX-201-046) (900ng), SUMO (10µg) in 50mM
TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase
and 0.6U/ml of inorganic pyrophosphatase. 10µg are sufficient for about 100 assays.
-80°C
